Villanueva-Ornelas GEa , Núñez-Anita REb , Arenas-Arrocena MCc, Luévano-Colmenero GHa,c, Acosta-Torres LSc
Figure 1: Molecular structure of chitosan, triclosan and type 1 collagen.
Table 1: Formulation of chitosan/triclosan/collagen matrixes
Figure 2: Shape and size of chitosan/triclosan/collagen matrixes obtained after lyophilization, a) matrix surface and shape; b) texture; c) matrix thickness.
Figure 3: Inhibition zone obtained in S. aureus cultures after 24 h of incubation: a) Amoxicillin/clavulanic acid, b) Ampicillin, c) Vancomycin, d) Group 1, e) Group 2, f) Group 3.
Figure 4: Comparison of the inhibition zones (radial distance) over a 24 h period using One-way ANOVA test (P <0.05), followed by a comparison between groups using Tukey post hoc test. *P≤0.05 represents significant difference.
Figure 5: Raman spectroscopy results for the three experimental chitosan/triclosan/collagen matrixes.
Figure 6: Results of FT-IR for the three experimental groups.
Table 2: Concentration of matrix residues in PBS/type 1 collagenase solution at 350 nm and percentage of mass loss of the matrixes at the following incubation times: 1, 4 and 7 days.
Figure 7: Viability of NIH-3T3 cells after 24-h treatment. Comparison of the means was analyzed by a One-way ANOVA test (P <0.05), followed by Tukey’s multiple comparison test (significance was set at p< 0.05. * represents significant difference.